RP-10

OSNA – One Step Nucleic Acid Amplification – is an automated rapid molecular diagnostic test for the detection of lymph node (LN) metastases. It analyses the entire LN tissue using a reverse transcriptase loop-mediated isothermal amplification reaction (RT-LAMP*) to quantify tumour-specific cytokeratin 19 (CK19) mRNA expression in LNs.

The pretreatment system RP-10 is an accessory to the OSNA automated molecular diagnostic test.

RP-10 is an instrument to homogenise lymph nodes prior to measurement by the Gene Amplification Detector RD-210. Homogenisation is performed using the reagent “LYNORHAG” in combination with the disposable “LYNOPREP BLADE SET”.

This instrument can homogenise up to 4 lymph node samples at the same time, taking about 1 minute 30 seconds from the start to the end of the homogenisation process.

Features

LYNOPREP BLADE SET (cat #AK186696) is an accessory for the OSNA automated molecular diagnostic test. Composed of containers and blades for the automated and effective homogenisation of lymph node tissues by the RP-10 pretreatment system.

LYNORHAG (cat #CL485270) is a buffer for the lysis of cells derived from mechanically homogenised human lymph node tissue. LYNORHAG is intended to be used for the preparation of samples from surgically removed lymph node(s) for use with the OSNA automated molecular diagnostic test. LYNORHAG is intended for use exclusively in association with the Gene Amplification Detector RD-210, pretreatment system RP-10 and LYNOAMP CK19 E.

Article number CL485270
Capacity 110 mL, 27 OSNA samples
Components Glycine 200 mmol/L
Dimethylsulfoxide 20%
Detergent 5%
Dimensions box W50 mm x D50 mm x H104 mm
Weight box total 145 g; bottle only 115 g
Storage conditions 2 to 8 °C
Shelf life 12 months after production
In-use expiry 2 months after opening

 

Technical Specifications
Article number BL085582
Throughput Max. 4 tests per run (90 s)
Dimension W391 mm x D267 mm x H500 mm
Weight ~23 kg
Reagents LYNORHAG
Disposables LYNOPREP BLADE SET
Parameter rpm

 

Contacts
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Sysmex Nordic ApS Suomen sivuliike

KPY Novapolis

Microkatu 1

70210 Kuopio

Finland

+358 102999940

Training

OSNA complete video training.

These trainings are intended for pathologists and everyone who operates the instrument.

Using RP-10 video

OSNA training videos

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RD-210

RD-210

OSNA gene amplification detector

Details
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